RAD50 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IF, IP, Simple Western, WB
Recommend Dilution	ICC/IF (Negative), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation: 1:10-1:500, Western Blot: 1:2000, Simple Western: 1:2000
Reactivity	Human, Mouse, Hamster
Host	Rabbit
Clonality	Polyclonal
Immunogen	C-terminal mouse RAD50 (604 amino acids). [UniProt# P70388]
Formulation	Store at 4C. Do not freeze.
Concentration	lot specific
Purification	Whole antisera
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	153 kDa
Gene Name	RAD50 double strand break repair protein
Database Link	NP_005723 Entrez Gene 19360 MouseEntrez Gene 10111 Human UniProt ID Q92878
Background	RAD50 (DNA repair protein RAD50) is a component of MRN complex (Mre11-Rad50-Nbs1) that plays an important role in detection and signaling of DNA double strand breaks (DSBs) through acting as DSB sensor, co-activator of DSB-induced cell cycle checkpoint signaling, and as repair-effector in two competing DSB repair pathways: homologous recombination (HR) and non-homologous end-joining (NHEJ). MRN complex also associates with telomeres at the ends of linear chromosomes, where it contributes to their maintenance. RAD50 is required to bind DNA ends for holding them in close proximity where it facilitates searches for short or long regions of sequence homology in the recombining DNA templates, and also stimulate the activity of DNA ligases and/or restrict MRE11A's nuclease activity to prevent nucleolytic degradation. DSBs can be caused by ionizing radiation, certain chemotherapy drugs, metabolic ROS, as errors during replication, by programmed enzymatic activities during meiosis/V(D)J recombination etc., and if left unrepaired, DSBs can generate chromosomal translocations, aneuploidy and carcinogenesis. Defects in RAD50 have been linked to Nijmegen breakage syndrome-like disorder (NBSLD).
Synonyms	hRad50; NBSLD; RAD502
Note	This RAD50 antibody is useful for Immunoprecipitation and Western blot, where a band can be seen at 153 kDa. IP has been done with 3-4 X 10(6) cells. ICC/IF does not appear to work. Immunocytochemistry/Immunofluorescence was reported in scientific literature.
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Homologous recombination, Non-homologous end-joining

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