B3galt5 Rat Monoclonal Antibody [Clone ID: MC-631]

Specifications

Product Data
Clone Name	MC-631
Applications	CyTOF-ready, FC, ICC/IF, IHC, IP, WB
Recommend Dilution	Western Blot: 1:500, Immunohistochemistry-Paraffin: 1:10-1:500, CyTOF-ready, Immunohistochemistry-Frozen: 1:10-1:500, Flow Cytometry: 10-20 ug/ml, Immunohistochemistry: 1:10-1:500, Immunocytochemistry/ Immunofluorescence: 1:50-1:100, Immunoprecipitation: 1:10-1:500
Reactivity	Human, Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	4-8 cell stage mouse embryos.
Formulation	Tris-glycine, 150mM NaCl pH 7.5, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	IgM purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5
Database Link	NP_001116465 Entrez Gene 10317 HumanEntrez Gene 93961 Mouse UniProt ID Q9JI67
Background	SSEA-3 (stage-specific embryonic antigen-3) is a member of SSEAs which are present on eggs as well as preimplantation embryos at specific stages of early development. SSEAs are glycan epitopes which resides on glycoproteins/glycolipids of mammalian cells, and the epitope of several of these antigens consists solely of sugar residues. SSEA-3 antigen was identified following immunization of rats with 4-8 cell mouse embryos and was present on glycolipids of human teratocarcinoma cells. During protein glycosylation, beta-1,3-galactosyltransferase 5 catalyzes the transfer of Gal to the terminal GalNAc unit of the globoside GB4, thereby synthesizing glycolipid GB5 which is SSEA-3. In its functions. SSEA-3 has been proposed to involve in the process of cell differentiation. SSEA3 expression is mostly restricted to the cytoplasm or apical surface of epithelial cells and it has been reported to express in various normal tissues with high expression levels in kidney. SSEA3 has been reported to be expressed in the ascites fluids from patients of hepatoma or pancreatic cancer, breast cancer including breast cancer stem cells.
Synonyms	b3Gal-T5; B3GalT-V; B3GalTx; B3T5; Beta-3-Gx-T5; beta3Gal-T5; GLCT5; OTTHUMP00000109186
Note	This SSEA3 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry frozen and paraffin sections, Immunoprecipitation and Western Blot.
Reference Data

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