EAAT1 (SLC1A3) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FC, ICC/IF, IHC, WB
Recommend Dilution	Flow (Intracellular): 1:500, Immunocytochemistry/ Immunofluorescence: 1:10-1:500, Immunohistochemistry: 1:10-1:500, Immunohistochemistry-Frozen: 1:10-1:500, ELISA: 1:100 - 1:2000, Flow Cytometry: 1:200-1:500, Immunohistochemistry-Paraffin: 1:50-1:500, Western Blot: 2 ug/ml
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to a C-terminal portion of the rat SLC1A3 protein (between residues 500-542) [UniProt P24942]
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	60 kDa
Gene Name	solute carrier family 1 member 3
Database Link	NP_001160167 Entrez Gene 20512 MouseEntrez Gene 29483 RatEntrez Gene 6507 Human UniProt ID P43003
Background	Excitatory amino acid transporter 1 (SLC1A3), also known as sodium-dependent glutamate/aspartate transporter 1, GLAST-1, solute carrier family 1 member 3, EAAT1, and EA6, transports and regulates L-glutamate, D-aspartate, and L-aspartate. SLC1A3 ends the glutamate postsynaptic actions by removing remaining glutamate from the cleft and by reducing extracellular glutamate. SLC1A3 is a member of a family of high affinity sodium-dependent transporter molecules that regulate neurotransmitter concentrations at the excitatory glutamatergic synapses of the mammalian central nervous system. In addition, SLC1A3 may also be important for the prevention of glutamate excitotoxicity. The SLC1A3 protein is located on the cell membrane. SLC1A3 is prominently expressed in the cerebellum, frontal cortex, hippocampus and basal ganglia and is also reported to be found in heart, placenta, lung and striated muscle. The SLC1A3 protein is subject to glycosylation and phosphorylation. SLC1A3 has two known isoforms (59.5 and 55 kDa) formed by alternative splicing. Mutations in the SLC1A3 gene have been linked to episodic ataxia and hemiplegia (PMID: 16116111) while other mutations result in a form of SLC1A3 (E219D) which influences Tourette syndrome (PMID: 21233784).
Synonyms	EA6; EAAT1; GLAST; GLAST1
Reference Data
Protein Families	Transmembrane

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