EGLN1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, IP, WB
Recommend Dilution	Immunohistochemistry-Paraffin: 2.5 - 5.0 ug/mL, Immunoprecipitation: 1:10 - 1:500, Immunocytochemistry/ Immunofluorescence: 1:50 - 1:500, Immunohistochemistry: 2.5 - 5.0 ug/mL, Western Blot: 2 ug/mL, Knockout Validated, Knockdown Validated
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to an internal portion of mouse PHD2/HIF Prolyl Hydroxylase 2 (between residues 300-400). [UniProt# Q91YE3]
Formulation	Tris-citrate/phosphate, pH 7, 0.1% Sodium azide. Store at 4C. Do not freeze.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	43 kDa
Gene Name	egl-9 family hypoxia inducible factor 1
Database Link	NP_071334 Entrez Gene 112405 MouseEntrez Gene 54583 Human UniProt ID Q9GZT9
Background	HIF prolyl 4-hydroxylases (PHDs) are proyl hydroxylase domain-containing enzymes (PHD1/ Egln2, PHD2/ Egln1, PHD3/ Egln3, and P4H-TM) that mediate important physiological responses to hypoxia by modulating HIF1alpha levels. The HIF-alpha is regulated by hydroxylation, both by a family of PHDs leading to ubiquitination and proteasomal degradation, and by transcriptional inactivation following asparaginyl hydroxylation by FIH (factor inhibiting HIF). Under normoxic conditions, HIF Prolyl Hydroxylase 2 (HIF1-PH; also called PHD1 or Egln2) catalyzes the post-translational formation of 4-hydroxyproline through hydroxylation of a specific proline found in each of NODD/CODD domains of HIF1A and also hydroxylates HIF2A with preference for CODD site of HIF1A/HIF1B. After hydroxylation, HIFs undergo proteasomal degradation via VHL (von Hippel-Lindau) ubiquitination complex. However, under hypoxic conditions, the hydroxylation reaction is tempered which allows HIFs to escape degradation process followed by their nuclear translocation, heterodimerization with HIF1B, and increased expression of hypoxia-inducible genes. HIF1-PH, through regulating HIF1 stability, is involved in various hypoxia-influenced processes such as angiogenesis in retinal/cardiac functionality as well as tumor angiogenesis, and defecticve EGLN1 have been linked to ECYT3 (familial erythrocytosis type 3).
Synonyms	C1orf12; ECYT3; HALAH; HIF-PH2; HIFPH2; HPH-2; HPH2; PHD2; SM20; ZMYND6
Note	This HIF Prolyl Hydroxylase 2 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin embedded sections and Immunoprecipitation. In Western blot a band is seen ~43 kDa representing HIF Prolyl Hydroxylase 2. There is also a non-specific band of similar intensity at ~75 kDa.
Reference Data
Protein Pathways	Pathways in cancer, Renal cell carcinoma

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