Cytochrome C (CYCS) Mouse Monoclonal Antibody [Clone ID: 7H8.2C12]

Specifications

Product Data
Clone Name	7H8.2C12
Applications	FC, ICC/IF, IHC, Immunoblotting, WB
Recommend Dilution	Western Blot: 0.05-0.5 ug/ml, Immunocytochemistry/ Immunofluorescence: 1:10-1:500, Simple Western: 2.5 ug/ml, Immunohistochemistry-Paraffin: 1:10-1:500, Flow Cytometry: 1:20-1:2000, Immunohistochemistry: 1:10-1:500, Flow (Intracellular)), Flow (Cell Surface), Immunoblotting
Reactivity	Human, Mouse, Canine, Drosophila, Equine, Mammalian
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon CYT were used as the immunogen (Jemmerson et al. 1991). The antibody recognizes an epitope within amino acids 93-104 of pigeon cytochrome C based on competitive ELISA result
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	cytochrome c, somatic
Database Link	NP_061820 Entrez Gene 13063 MouseEntrez Gene 54205 Human UniProt ID P99999
Background	In addition to its well-known role in electron transport, cytochrome c (CYT) has been identified as a mediator of apoptosis or programmed cell death. Cytochrome c translocates from mitochondria into the cytoplasm in the early phases of apoptosis and plays a role in initiation of the apoptotic proteolytic cascade by activating caspase-3. Cytochrome c has been shown to bind Apaf-1. Apaf-1 has also been shown to activate capase-3.
Synonyms	CYC; HCS; THC4
Note	Useful in Flow Cytometry (Intracellular): See Mohr (2004) et al. Immunocytochemistry/Immunofluorescence: See Yamasaki (2006) et al. Immunohistochemistry-Paraffin: See Fujimara (1998). et al.
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Huntington's disease, p53 signaling pathway, Parkinson's disease, Pathways in cancer, Small cell lung cancer, Viral myocarditis

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