IKB alpha (NFKBIA) Mouse Monoclonal Antibody [Clone ID: 6A920]

Specifications

Product Data
Clone Name	6A920
Applications	CyTOF-ready, FC, ICC/IF, IHC, IP, Simple Western, WB
Recommend Dilution	Flow Cytometry: 0.25-1 ug/10^6 cells, Immunoprecipitation: 1 ug/ml, Western Blot: 1-2 ug/ml, Immunohistochemistry: 1:20-1:1000, Simple Western: 1:20, Immunohistochemistry-Paraffin: 1:100, Immunocytochemistry/ Immunofluorescence: 1:100-1:1000, Knockout Validated, CyTOF-ready
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	A recombinant protein corresponding to amino acid residues 32-291 of human IkBa was used as immunogen.
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	NFKB inhibitor alpha
Database Link	NP_065390 Entrez Gene 18035 MouseEntrez Gene 25493 RatEntrez Gene 4792 Human UniProt ID P25963
Background	NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first gene induced following NF-kB activation is IkBa.
Synonyms	IKBA; MAD-3; NFKBI
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway

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