RANK (TNFRSF11A) Mouse Monoclonal Antibody [Clone ID: 9A725]

Specifications

Product Data
Clone Name	9A725
Applications	CyTOF-ready, FC, IHC, WB
Recommend Dilution	Immunohistochemistry: 1:10-1:500, Immunohistochemistry-Paraffin: 1:10-1:500, Flow (Cell Surface), Western Blot: 1-2 ug/ml, Flow Cytometry: 2-4 ug/ml, CyTOF-ready
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	A fusion protein containing amino acid residues 326-616 of human RANK was used as immunogen. This antibody recognizes an epitope located between amino acid residues 330-427 of human RANK (NP_003830).
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	tumor necrosis factor receptor superfamily member 11a
Database Link	NP_003830 Entrez Gene 21934 MouseEntrez Gene 8792 Human UniProt ID Q9Y6Q6
Background	Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Receptor activator of NF-kB (RANK) is a recently cloned member of the TNFR superfamily with no significant homology to other members of this family. RANK ligand (RANKL/TRANCE/ OPGL) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival . The cytoplasmic domain of RANK interacts with TRAF2, TRAF5 and TRAF6. Overexpression of RANK activates NF-kB and c-Jun-terminal kinase (JNK) pathways. Recent studies have shown that RANK interaction with TRAF6 activates NF-kB, whereas JNK activation is mediated through binding of RANK to TRAF2.
Synonyms	CD265; FEO; LOH18CR1; ODFR; OFE; OPTB7; OSTS; PDB2; RANK; TRANCER
Note	Bharti et al (2004) and Reigel et al (2012) both published the RANK antibody clone 9A725 for Flow (Cell Surface). However, the epitope range recognized by this antibody is a potential cytoplasmic region as defined by www.uniprot.org. Hence, researchers are encouraged to refer to both the publication and the bioinformatics data bases for additional information in order to make their own determination regarding the suitability of the antibody for cell surface flow cytometry.
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Cytokine-cytokine receptor interaction

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