Myogenin (MYOG) Mouse Monoclonal Antibody [Clone ID: 5FD]

Specifications

Product Data
Clone Name	5FD
Applications	ICC/IF, IHC, WB
Recommend Dilution	Western Blot: 2 ug/mL, Immunocytochemistry/ Immunofluorescence: 5 ug/mL, Immunohistochemistry: 1:20 - 1:1000, Immunohistochemistry-Frozen: 10 - 20 ug/mL, Immunohistochemistry-Paraffin: 20 - 40 ug/mL
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	A recombinant protein corresponding to amino acids 30-224 of rat myogenin was used as immunogen (NP_002470).
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	myogenin (myogenic factor 4)
Database Link	NP_002470 Entrez Gene 17928 MouseEntrez Gene 29148 RatEntrez Gene 4656 Human UniProt ID P15173
Background	Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Transfection of myogenin and other family members into a variety of non-muscle cells has been shown to either convert these cells to myogenic cells, or to transcriptionally activate a set of otherwise unexpressed muscle-specific genes. In addition to activating muscle specific genes, members of the MyoD family members activate their own transcription and transactivate the transcription of other MyoD family members. For example, transfection of myogenin into 10T1/2 cells or Swiss 3T3 cells results in the activation of theendogenous myogenin gene as well as transactivation of MyoD. Likewise, the transfection of MyoD into these cells results in the activation of MyoD as well as the transactivation of myogenin. Each member of the MyoD family has distinct roles in muscle development; myogenin plays a key role in muscle maturation. Myogenin migrates at a molecular weight of approx. 34 kDa by SDS-PAGE.
Synonyms	bHLHc3; myf-4; MYF4
Reference Data
Protein Families	Druggable Genome, Transcription Factors

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.