DAP3 Mouse Monoclonal Antibody [Clone ID: 42C617.1.2]

Specifications

Product Data
Clone Name	42C617.1.2
Applications	WB
Recommend Dilution	Western Blot: 3-5 ug/ml
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	The antibody was raised against a synthetic peptide corresponding to amino acids 51-66 of human DAP-3 protein.
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	death associated protein 3
Database Link	NP_387506 Entrez Gene 65111 MouseEntrez Gene 7818 Human UniProt ID P51398
Background	Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Recently, five novel apoptotic genes (named DAP genes for: Death Associated Proteins) have been identified that code for proteins, which display a diverse spectrum of biochemical activities. These include a novel type of calcium/calmodulin-regulated kinase which carries ankyrin repeats and a death domain (DAP-kinase), a nucleotide-binding protein (DAP-3), a small proline-rich cytoplasmic protein (DAP-1), and a novel homolog of the eIF4G translation initiation factor (DAP-5). DAP-3 has an ATP/GTP binding motive (P-loop) and has been isolated from human and mouse, and named as hDAP-3 and mDAP-3 (Death Associated Protein-3), respectively. Sequence analysis revealed an 82% amino acid identity between human and mouse protein. In situ hybridization and northern blot analysis showed an abundant mRNA expression with a pronounced expression in highly proliferative epithelial compartments. Overexpression of DAP-3 leads to cytochrome c release and induction of cell death. DAP-3 mediated apoptosis was shown to depend on a functional P-loop. mDAP-3 is localized to the mitochondrial matrix and in contrast to cytochrome c, retained its mitochondrial localization during apoptosis induction.
Synonyms	bMRP-10; DAP-3; MRP-S29; MRPS29
Reference Data
Protein Families	Druggable Genome

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