PARP1 Mouse Monoclonal Antibody [Clone ID: 194C1439]

Specifications

Product Data
Clone Name	194C1439
Applications	FC, ICC/IF, WB
Recommend Dilution	Western Blot: 1 - 2 ug/mL, Flow Cytometry, Flow (Intracellular): 1 - 10 ug/1x10^6 cells, Immunocytochemistry/ Immunofluorescence: 2-5 ug/ml
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	This antibody was developed by immunizing mice with a synthetic peptide containing amino acids near 214/215-cleavage site of human PARP.
Formulation	PBS containing 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	poly(ADP-ribose) polymerase 1
Database Link	NP_001609 Entrez Gene 142 Human UniProt ID P09874
Background	The poly (ADP-ribose) polymerase (PARP) is involved in cell recovery from DNA damage, such as methylation of N3-adenine, which activates the base excision repair process. PARP [Poly (ADP-ribose) polymerase] is a 116 kDa nuclear chromatin-associated enzyme that is cleaved during apoptosis by caspase-3 into a 24 kDa fragment containing the DNA binding domain and an 89 kDa fragment containing the catalytic and automodification domains. The 24 kDa-fragment irreversibly bind to DNA and may contribute to the irreversibility of apoptosis by blocking the access of DNA repair enzymes to DNA strand breaks.
Synonyms	ADPRT; ADPRT 1; ADPRT1; ARTD1; pADPRT-1; PARP; PARP-1; PPOL
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency, Transcription Factors
Protein Pathways	Base excision repair

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