p73 (TP73) Mouse Monoclonal Antibody [Clone ID: 5B1288]

Specifications

Product Data
Clone Name	5B1288
Applications	ChIP, ICC/IF, IHC, IP, WB
Recommend Dilution	Chromatin Immunoprecipitation: 1:20 - 1:1000, Western Blot: 1-3 ug/ml, Immunohistochemistry-Paraffin: 1:100 - 1:200, Immunohistochemistry: 1:100 - 1:200, Chromatin Immunoprecipitation (ChIP): 1:20-1:1000, Immunoprecipitation: 1:20 - 1:1000, Immunocytochemistry/ Immunofluorescence: 1:10-1:2000
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	This antibody was raised against full-length human p73. The epitope is thought to lie around the center of the molecule (NP_005418).
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at -20C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	73 kDa
Gene Name	tumor protein p73
Database Link	NP_005418 Entrez Gene 7161 Human UniProt ID O15350
Background	p73 was identified as a long-lost cousin of tumor suppressor protein, p53. p73 has high homology with p53 as well as with p63, a gene implicated in the maintenance of epithelial stem cells. Significant homology between p53, p63, and p73 (approximately 63% amino acid identity in the DNA-binding domain suggest that they may have overlapping functions in the regulation of gene expression. The targeted disruption of p73 gene leads to defects hippocampal dysgenesis, hydrocephalus, chronic inflammation and infections. Recently, spilicing variant mRNAs of p73 has been identified in MCF-7, a breast carcinoma cell line. These mRNAs code for variant p73 proteins bearing distinct carboxy-terminal structures suggesting that the carboxy-terminal region of p73 may be important for the functions of this protein. Tumor BioMarker: Vella et al (2003) found p73 to be upregulated in a significant fraction of anaplastic thyroid cancers, whereas p73 was not detectable in normal thyroid epithelial cells nor in papillary or follicular thyroid cancer.
Synonyms	P73
Note	Reported use for Chromatin Immunoprecipitation assay (See accardi et al); Immunocytochemistry/Immunofluorescence and Immunoprecipitation (see Sayan et al)
Reference Data
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Neurotrophin signaling pathway, p53 signaling pathway

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