IKK alpha (CHUK) Mouse Monoclonal Antibody [Clone ID: 14A231]

Specifications

Product Data
Clone Name	14A231
Applications	ChIP, FC, ICC/IF, IHC, IP, WB
Recommend Dilution	Flow Cytometry: (Intracellular): 0.25-0.5 ug/10^6 cells, Chromatin Immunoprecipitation (ChIP): 1:10-1:500, Western Blot: 1 ug/ml, Simple Western: 10 ug/ml, Immunoprecipitation: 1-2 ug/ml, Flow (Intracellular): Flow Cytometry: (Intracellular): 0.25-0.5 ug/10^6 cells, Immunohistochemistry-Frozen, Chromatin Immunoprecipitation: 1:10 - 1:500, Immunohistochemistry: 1:200, Immunohistochemistry-Paraffin: 5ug/ml, Immunocytochemistry/ Immunofluorescence: 1:10
Reactivity	Human, Mouse, Primate
Host	Mouse
Clonality	Monoclonal
Immunogen	This antibody was raised against a His-tagged full-length human IKK alpha protein.
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	conserved helix-loop-helix ubiquitous kinase
Database Link	NP_001269 Entrez Gene 12675 MouseEntrez Gene 1147 Human UniProt ID O15111
Background	NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. In vitro, IKK1/a and IKK2/b can form homo- and heterodimers that can phosphorylate IkBs at the regulatory serine residues directly. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Targeted disruption of IKK1/a gene in mice results in skin and limb abnormalities and death of newborns.
Synonyms	IKBKA; IKK-alpha; IKK1; IKKA; NFKBIKA; TCF16
Note	Use in Flow Intracellular reported in scientific literature (PMID 24804954)
Reference Data
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, MAPK signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway

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