TBK1 Mouse Monoclonal Antibody [Clone ID: 108A429]

Specifications

Product Data
Clone Name	108A429
Applications	ICC/IF, IHC, IP, WB
Recommend Dilution	Immunocytochemistry/ Immunofluorescence: 1:20, Immunohistochemistry: 1:10-1:500, Immunohistochemistry-Paraffin: 1:10-1:500, Western Blot: 1-2 ug/ml, Simple Western: 10 ug/ml, Knockout Validated, Immunoprecipitation
Reactivity	Human, Mouse, Rat, Bovine, Canine
Host	Mouse
Clonality	Monoclonal
Immunogen	A synthetic peptide corresponding to amino acids 563-577 (YYQFKKDKAERRLAYC) of human TBK1 was used as immunogen for this antibody (NP_037386).
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	TANK binding kinase 1
Database Link	NP_037386 Entrez Gene 56480 MouseEntrez Gene 299827 RatEntrez Gene 29110 Human UniProt ID Q9UHD2
Background	NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Recently, a new IKK family member, TBK1 [(TANK-binding kinase), also known as NAK and T2K] has been identified. TANK (TRAF-associated NF-kB activator) interacts with TBK1 and activates NF-kB in a kinase dependent manner.
Synonyms	NAK; T2K
Note	In Western Blot this antibody detects a band at 80 kDa.
Reference Data
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway

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