NUR77 (NR4A1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ChIP, ICC/IF, IHC, IP, WB
Recommend Dilution	Chromatin Immunoprecipitation (ChIP): 1:20-1:1000, Western Blot: 1:500-1:1000, Immunohistochemistry-Paraffin: 1:200, Chromatin Immunoprecipitation: 1:20-1:1000, Immunocytochemistry/ Immunofluorescence: 1:10-1:2000, Immunoprecipitation: 1:20-1:1000, Immunohistochemistry: 1:200
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	This antibody was developed against a synthetic peptide corresponding to aa 251-266 of human Nak1 (NP_775180).
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	nuclear receptor subfamily 4 group A member 1
Database Link	NP_002126 Entrez Gene 15370 MouseEntrez Gene 3164 Human UniProt ID P22736
Background	Nak1 is a member of the steroid/thyroid hormone receptor superfamily. The gene for Nak-1 is induced rapidly by androgens/growth factors and may have functions related to cell proliferation, differentiation and apoptosis (1). Liu et al (2) have demonstrated that mouse nur77 is necessary for induced apoptosis in T-cell hybridomas and can also be induced during early mitogenesis. Nak1 is a phosphoprotein and its size ranges from 67 kD to 88 kD, depending on post-translational modifications.
Synonyms	GFRP1; HMR; N10; NAK-1; NGFIB; NP10; NUR77; TR3
Note	Chromatin Immunoprecipitation: See Lee et al (2009) Immunocytochemistry/Immunofluorescence: See Chintharlapalli et al (2005) Immunoprecipitation: See Ferlini et al (2009)
Reference Data
Protein Families	Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
Protein Pathways	MAPK signaling pathway

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