HIF-1 alpha (HIF1A) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, Simple Western, WB
Recommend Dilution	Knockdown Validated, Immunocytochemistry/ Immunofluorescence: 1:10 - 1:500, Immunohistochemistry-Paraffin: 1:100 - 1:300, Immunohistochemistry-Frozen: 1:100 - 1:300, Western Blot: 1:1000, Simple Western: 1:100, Immunohistochemistry: 1:100 - 1:300, Gel Super Shift Assays
Reactivity	Human, Mouse, Rat, Porcine
Host	Rabbit
Clonality	Polyclonal
Immunogen	Fusion protein containing amino acids 432-528 of human HIF-1 alpha [UniProt# Q16665]
Formulation	PBS, 0.01% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	hypoxia inducible factor 1 alpha subunit
Database Link	NP_851397 Entrez Gene 15251 MouseEntrez Gene 29560 RatEntrez Gene 3091 Human UniProt ID Q16665
Background	HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (HIF-1 alpha and HIF-1 beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha protein is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIF1s regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions.
Synonyms	bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8
Note	This HIF-1 alpha antibody is useful for Western blot. Nuclear extracts are recommended. Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen and Immunohistochemistry-Paraffin were reported in scientific literature.
Reference Data
Protein Families	Transcription Factors
Protein Pathways	mTOR signaling pathway, Pathways in cancer, Renal cell carcinoma

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