LYVE1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, IHC, WB
Recommend Dilution	Flow (Intracellular), Western Blot: 1:500-1:2000, Immunohistochemistry-Paraffin: 1:100-1:200, Immunohistochemistry-Frozen: 1:100-1:200, Flow Cytometry: 1:50-1:500, Immunohistochemistry: 1:100-1:200
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to a C-terminal portion of the mouse LYVE1 protein sequence (between residues 250-318). [UniProt# Q8BHC0]
Formulation	PBS, 0.02% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	45 kDa
Gene Name	lymphatic vessel endothelial hyaluronan receptor 1
Database Link	NP_006682 Entrez Gene 114332 MouseEntrez Gene 293186 RatEntrez Gene 10894 Human UniProt ID Q9Y5Y7
Background	LYVE1 (lymphatic vessel endothelial hyaluronan receptor-1) is the lymphatic vessel endothelial hyaluronic acid receptor located in lymph nodes and in the luminal/abluminal surfaces of lymphatic vessels. It is one of the most specific and widely used mammalian lymphatic endothelial markers. LYVE-1 exists as disulfide-linked homodimer, can interact with PDGFB/IGFBP3 and also forms transient ternary complex with PDGFB and PDGFRB in trans-Golgi network (TGN). LYVE1 is a single-pass type I membrane protein localized in plasma membrane and in vesicles near extranuclear membranes which represents TGN and endosomes or pre-lysosomeal compartments. LYVE1 undergoes ligand-dependent internalization and recycling at the cell surface, and executes ligand-specific transporter trafficking between intracellular organelles/TGN and plasma membrane. LYVE1 plays a key role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding. LYVE1 behaves as hyaluronan transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes. LYVE-1 is very efficient in the passage of lymphocytes as well as the tumor cells into the lymphatics.
Synonyms	CRSBP-1; HAR; LYVE-1; XLKD1
Note	This LYVE1 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin and Western Blot where a band at ~45 kDa is seen. For Immunohistochemistry citrate buffer antigen retrieval is recommended.
Reference Data
Protein Families	Druggable Genome, Transmembrane

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