Hormone sensitive lipase (LIPE) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	Western Blot: 1:100-1:2000
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A fusion protein containing an internal portion of the rat HSL protein (between residues 699-908). [Swiss-Prot P15304]
Formulation	PBS containing 1 mg/ml BSA, 0.05% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	IgG purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	lipase E, hormone sensitive type
Database Link	NP_005348 Entrez Gene 16890 MouseEntrez Gene 25330 RatEntrez Gene 3991 Human UniProt ID Q05469
Background	HSL (hormone-sensitive lipase) is a key enzyme in lipid metabolism regulation in several tissues and this intracellular neutral lipase hydrolyzes triacylglycerols (TG), diacylglycerols (DG), and monoacylglycerols, as well as cholesterol and retinyl esters. While ATGL selectively performs the first step in TG hydrolysis, HSL is the most active enzyme against DG, which gets hydrolyzed faster than TG. HSL has been shown to express in several non-adipose tissues, including ovary, adrenal gland, heart, skeletal and smooth muscle, placenta, and pancreatic beta-cells, as well as testes. Under basal conditions, HSL is exclusively found in the cytosol of adipocytes, whereas upon phosphorylation mediated activation via PKA, HSL translocates from cytosol to lipid droplet surfaces for participation in lipolysis. HSL activity is regulated primarily by serine phosphorylation, which is stimulatory at Ser-563/Ser-659 via PKA and inhibitory at Ser-565 via AMPK. Mice lacking HSL have decreased nonesterified fatty acid (NEFA) and increased cholesterol concentrations in plasma and are resistant to diet-induced obesity. HSL has emerged as a potential target for lipid disorders and obesity.
Synonyms	AOMS4; FPLD6; HSL; LHS
Note	This HSL antibody is useful for Western Blot, where a band at ~84kDa is seen representing HSL protein. A non-specific band may be seen at ~89 kDa. Bands at ~102, 113, and 127 kDa may also be seen in the testis of sexually immature rats.
Reference Data
Protein Pathways	Insulin signaling pathway

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