DISC1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC
Recommend Dilution	Immunohistochemistry-Frozen, Immunohistochemistry: 2.5-5.0 ug/ml, Immunocytochemistry/ Immunofluorescence: 1:100, Immunohistochemistry-Paraffin: 2.5-5ug/ml
Reactivity	Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to an internal region (within residues 700-800) of the mouse DISC1 protein. [Swiss-Prot# Q811T9]
Formulation	Tris-glycine, 150 mM NaCl, 0.05% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	disrupted in schizophrenia 1
Database Link	NP_061132 Entrez Gene 244667 MouseEntrez Gene 307940 Rat UniProt ID Q9NRI5
Background	DISC1 (Disrupted in Schizophrenia 1) is a well known risk genes for psychiatric disorders including schizophrenia, major depression, bipolar disorder, and autism. DISC1 protein localize to cytoskeleton-rich regions in cells, including the centrosome, base of primary cilia, axon and dendritic shafts and spines. For serving its multiple functions, DISC1 interacts with CCDC88A, GSK3B, PCNT, tubulin alpha, ACTN2, ANKHD1, ATF4, ATF5, CEP63, EIF3S3, MAP1A, NDEL1, PAFAH1B1, RANBP9, SPTBN4, SYNE1, TRAF3IP1, CHCHD6 and NDEL1. DISC1 interaction with microtubules TRAF3IP1. In neurogenesis regulation, DISC1 is essential for neural progenitor proliferation in the ventrical/subventrical zone during embryonic brain development as well as in adult dentate gyrus of hippocampus. DISC1 modulates GSK3Beta activity and CTNNB1 abundance for acting as positive regulator of WNT-mediated neural progenitor proliferation. DISC1 can modulate AKT-mTOR signaling for controlling the neuron positioning, dendritic development and synapse formation which ultimately regulate newborn neurons integration during adult neurogenesis and can regulate the migration of early-born granule cell precursors toward the dentate gyrus during the hippocampal development. Moreover, DISC1 is implicated in microtubule network formation, regulation of functional biology of centrosome as well as primary cilia, neuronal migration and microtubule mediated transport.
Synonyms	C1orf136; SCZD9
Note	This DISC1 antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry paraffin embedded sections. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24560582) The theoretical molecular weight of DISC1 is ~90 kDa. Preliminary Western Blot studies have been performed with this antibody, where several non-specific bands are seen. Due to alternative splicing, DISC1 may also run at ~75 and 100 kDa. In ICC/IF, cytoplasmic staining was observed in PC12 cells.
Reference Data

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