LOX Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, Immunoblotting, WB
Recommend Dilution	Western Blot: 1:500-1:2000, Immunohistochemistry: 0.5-1.0 ug/ml, Immunohistochemistry-Paraffin: 1:100-1:250, Immunocytochemistry/ Immunofluorescence: 5 ug/ml, Immunoblotting
Reactivity	Human, Mouse, Rat, Porcine, Primate
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to an internal region of mouse LOX propeptide (residues 78-115). [UniProt# P28301]
Formulation	PBS, 0.02% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	lysyl oxidase
Database Link	NP_002308 Entrez Gene 16948 MouseEntrez Gene 24914 RatEntrez Gene 4015 Human UniProt ID P28300
Background	Lysyl oxidase (LOX) is a critical enzyme in extracellular matrix biosynthesis as well as in the maintainance of normal bone phenotype. It is synthesized/secreted as a 50-kD proenzyme 'pro-LOX' which is then processed to 30-kD mature enzyme 'LOX' and 18-kD lysyl oxidase propeptide 'LOX-PP' by extracellular procollagen C-proteinases (BMP1, Tll1, and Tll2). LOX-PP has been proposed to implicate in the maintenance of inactive state of LOX within the secretory pathway and to act as intramolecular chaperones which facilitate the accurate protein folding/targeting to correct destinations. LOX-PP involves tumor suppression by inhibiting RAS-dependent cellular transformation and its expression has also been associated with inhibition of anchorage-independent progression as well as migration and growth suppression xenografts in Her-2/neu-driven breast cancer. LOX-PP also inhibits PI3K/AKT and ERK1/2 MAP kinase pathways, as well as the levels of downstream NF-kB/cyclin D1 signals in certain cancers - breast, pancreatic, lung, prostate and oral cancers. LOX-PP has also been shown to inhibit DNA synthesis in rat's VSMCs cultures, osteoblast proliferation and FGF-2 signaling.
Synonyms	MGC105112
Note	This LOX propeptide antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin and Western Blot where a band is seen at ~18 kDa. The strong band at 50 kDa and smear of bands that run between 46 kDa and 50 kDa are glycosylated and non-glycosylated forms of pro-lysyl oxidase. Bands between 28-36 kDa are glycosylated propeptide. The variation in MW depends on the extent of glycosylation.
Reference Data
Protein Families	Druggable Genome, Secreted Protein

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