ATG9A Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, ICC/IF, IHC, IP, WB
Recommend Dilution	Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin: 1:100-1:250, Western Blot: 2 ug/ml, Flow Cytometry, Immunohistochemistry: 1:100-1:250
Reactivity	Human, Mouse, Rat, Bovine, Chicken, Primate
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to a region within the C-terminus of human ATG9A (between residues 750-839). [Swiss-Prot# Q7Z3C6].
Formulation	PBS, 30% glycerol, 0.1% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	95 kDa
Gene Name	autophagy related 9A
Database Link	NP_076990 Entrez Gene 245860 MouseEntrez Gene 363254 RatEntrez Gene 79065 Human UniProt ID Q7Z3C6
Background	ATG9A (autophagy-related protein 9A) is the only transmembrane member identified among the classical ATG proteins family which are essential to autophagy. Yeast ATG9 protein localizes to the pre-autophagosomal structure (PAS) as well as cytoplasmic punctate structures, whereas, the mammalian ATG9A localizes to endocytic compartments, the trans-Golgi network and autophagic membranes. Moreover, ATG9 is recruited to the PAS in an ATG11-dependent manner under nutrient-rich conditions, whereas during starvation, recruitment of ATG9 depends on Atg17p. ATG9A does not steadily reside at one place in cells, but rather dynamically shuttles between a juxta-nuclear trans-Golgi network compartment and late endosomes under autophagic or starvation conditions. Moreover, ATG9A accumulation is upregulated upon starvation in autophagosomes and the starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H. ATG9A interacts with SUPT20H and has been proposed to mediate membrane transport to generate autophagosomes.
Synonyms	APG9L1; mATG9; MGD3208
Note	This ATG9A antibody is useful for Immunohistochemistry paraffin embedded sections and Western blot. In Western blot this antibody recognizes a band at ~95 kDa, representing ATG9A protein. Boiling samples prior to running the gel may affect the protein. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24705551)
Reference Data
Protein Families	Transmembrane

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