ATG16L1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	Electron Microscopy, IHC, WB
Recommend Dilution	Electron Microscopy: 1:10-1:500, Western Blot: 0.5-2 ug/ml, Immunohistochemistry: 1:250 - 1:500, Immunohistochemistry-Paraffin
Reactivity	Human, Mouse, Rat, Bovine, Canine, Primate
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide within residues 1-100 of human ATG16L1 protein. [Swiss-Prot# Q676U5]
Formulation	PBS, 0.02% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	68 kDa
Gene Name	autophagy related 16 like 1
Database Link	NP_110430 Entrez Gene 77040 MouseEntrez Gene 363278 RatEntrez Gene 55054 Human UniProt ID Q676U5
Background	Atg16L1 (autophagy-related protein 16-1) forms a component of a protein complex that directs LC3 to autophagosomes en route to their fusion with lysosomes, thereby playing a key role in the process of autophagosome maturation. Atg16L1 belongs to WD repeat ATG16 family, exist as homooligomer and interacts with ATG5 as well as RAB33B, and forms a part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kD) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kD). It is found in cytoplasm and gets localized to preautophagosomal structure where it is implicated in the membrane targeting of ATG5. Mice deficient in Atg16L1 (Atg16L1HM) display reduced autophagy and develop, on viral infection, intestinal abnormalities similar to pathologies found in Crohn's disease patients, including abnormalities in the integrity, architecture, and function of Paneth cells, which are specialized secretory epithelial cells of the small intestine. Atg16L1 has also been shown to play a role in modulating proinflammatory responses and population genetic studies have positively linked a common polymorphism in ATG16L1 gene with inflammatory bowel disease type 10 (IBD10).
Synonyms	APG16L; ATG16A; ATG16L; IBD10; WDR30
Note	In Western blot analysis, a specific band is seen at ~68kDa. Electron Microscopy and IHC-P applications are reported in scientific literature (PMIDs 22531915 and 25060858 respectively).
Reference Data

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