FTO Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, Immunoblotting, WB
Recommend Dilution	Immunoblotting, Immunohistochemistry, Western Blot: 0.5 ug/ml
Reactivity	Human, Mouse, Primate
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide within the C-terminal region (residues 400-505) of the human FTO protein. [Swiss-Prot# Q9C0B1]
Formulation	PBS, 0.02% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	60 kDa
Gene Name	fat mass and obesity associated
Database Link	NP_001073901 Entrez Gene 26383 MouseEntrez Gene 79068 Human UniProt ID Q9C0B1
Background	FTO (alpha-ketoglutarate-dependent dioxygenase FTO) is a novel protein belonging to FTO family and it is a nuclear dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation reactions. FTO exerts highest demethylase activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine, low activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine and is inactive towards 1-methylguanine or double-stranded DNA. FTO requires O2, alpha-ketoglutarate and iron (co-factor) for its activity and gets activated by ascorbate whereas its inhibitors include N-oxalylglycine, fumarate, succinate etc. It contributes to the regulation of global metabolic rate, energy expenditure/homeostasis and has been suggested to control the body size as well as accumulation of body fat. FTO has been associated with various obesity traits, insulin resistance and NIDDM, and its defects are known to cause growth retardation developmental delay coarse facies and early death (GDFD).
Synonyms	ALKBH9; BMIQ14; GDFD
Note	This FTO antibody is useful for Western blot analysis, where a band is seen at ~60 kDa.
Reference Data

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