Beclin 1 (BECN1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, Simple Western, WB
Recommend Dilution	Immunocytochemistry/ Immunofluorescence: 1:50, Knockdown Validated, Simple Western: 1:12.5, Immunohistochemistry: 1:50 - 1:400, Immunohistochemistry-Paraffin: 1:50 - 1:400, Western Blot: 1:1000
Reactivity	Human, Mouse, Canine, Porcine, Primate
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide made to an internal portion of human Beclin 1 (within residues 150-300). [Swiss-Prot# Q14457]
Formulation	PBS, 0.02% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	51 kDa
Gene Name	beclin 1
Database Link	NP_003757 Entrez Gene 56208 MouseEntrez Gene 8678 Human UniProt ID Q14457
Background	Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic-lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl-2-binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. Beclin 1 encodes an evolutionarily conserved 60 kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons. In gene transfer studies, Beclin 1 promotes nutrient deprivation-induced autophagy, inhibits mammary tumorigenesis, and inhibits viral replication. Expression of the Beclin 1 protein is frequently decreased in malignant breast epithelial cells. Based upon these observations, it is speculated that beclin 1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections. Beclin 1 may also play a role in other biological processes in which autophagy is important such as cell differentiation and nutritional stress responses.
Synonyms	ATG6; beclin1; VPS30
Note	This Beclin 1 antibody is useful for Immunohistochemistry paraffin sections, Immunocytochemistry/Immunofluorescence, and Western blot where a band is seen ~51 kDa. Immunoprecipitation was reported in scientific literature.
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Regulation of autophagy

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