Rad9 (RAD9A) Mouse Monoclonal Antibody [Clone ID: 93A535]

Specifications

Product Data
Clone Name	93A535
Applications	ICC/IF, IHC, IP, WB
Recommend Dilution	Immunocytochemistry/ Immunofluorescence: 1:10-1:500, Immunohistochemistry-Paraffin: 1:10-1:500, Western Blot: 1-2 ug/ml, Immunohistochemistry: 1:10-1:500, Immunoprecipitation: 1:10-1:500
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	The antibody was raised against fusion protein of human full length Rad9.
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	RAD9 checkpoint clamp component A
Database Link	NP_004575 Entrez Gene 5883 Human UniProt ID Q99638
Background	Rad9 is a recently isolated human protein that is involved in the control of cell cycle checkpoint. Human Rad9 (hRad9) is homologue of the S. pombe Rad9. Six Rad proteins (Rad1, Rad3, Rad9, Rad17, Rad26, and Hus1) are involved in the early steps of DNA damage check point response in S. pombe. Recently, it has been shown that hRad9 interacts with Bcl-2 family proteins Bcl-2 and Bcl-XL, but not with BAX or BAD. Over-expression of hRad9 in mammalian cells leads to apoptosis, where as antisense Rad9 suppresses cell death induced by DNA damaging agents such as methanosulphonate. Human Rad9 undergoes complex post-translational modifications in undamaged cells and is hyperphosphorylated in response to DNA damage and makes ternary complex with human Rad1 and human HUS1 proteins. This may account for detection of multiple protein bands in some cases by western blot or immunoprecipitation analysis using anti-hRad9 antibody. HRad9 has an intrinsic 3-5 exonuclease activity suggesting that by interaction with DNA it may play a role in the damage-sensing step of the DNA damage checkpoint pathway.
Synonyms	RAD9
Note	Immunocytochemistry/Immunofluorescence: see Kotmatsu et al. Immunohistochemistry-Paraffin: see Lee et al (2007) for details.
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency

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