LIMPII (SCARB2) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, IP, Simple Western, WB
Recommend Dilution	Immunohistochemistry: 1:200, Immunocytochemistry/ Immunofluorescence: 1:50-1:250, Immunoprecipitation: 1:100, Western Blot: 1:1000, Simple Western, Immunohistochemistry-Paraffin
Reactivity	Human, Mouse, Rat, Canine, Primate
Host	Rabbit
Clonality	Polyclonal
Immunogen	A C-terminal synthetic peptide made to the mouse LIMPII/lgp85 protein sequence. [UniProt# O35114]
Formulation	Tris-citrate/phosphate, pH 7, 0.1% Sodium azide. Store at 4C. Do not freeze.
Concentration	lot specific
Purification	Whole antisera
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	85 kDa
Gene Name	scavenger receptor class B member 2
Database Link	NP_005497 Entrez Gene 12492 MouseEntrez Gene 117106 RatEntrez Gene 950 Human UniProt ID Q14108
Background	LIMP II (lysosome membrane protein 2; also called LGP85, SCARB2, CD36 antigen-like 2/CD36L2) is a member of the scavenger receptor class B member 2 family, ubiquitously expressed in higher organisms as a major lysosomal and endosomal membrane protein. LIMP II localize primarily to late endosomes/lysosomes as type III transmembrane protein, which traverses the membrane twice with the uncleaved signal peptide and a hydrophobic region near to the COOH-terminus, followed by twenty-amino acid long cytoplasmic tail of which a large portion located in lysosomal lumenal side possess 10-11 N-glycosylation sites. LIMP II acts as a lysosomal receptor for glucosylceramidase (GBA) targeting and it plays a key role in regulating the biogenesis of the endosomal/lysosomal compartments (ELCs). LIMP II serves as one of the major components of the molecular machinery required for intercompartmental membrane trafficking along the endocytic pathway rather than solely serving as a structural constituent of late endosomes and lysosomes. LIMP II deficiency has been found to result in the development of an obstruction of ureteric pelvic junction, deafness and peripheral neuropathy.
Synonyms	AMRF; CD36L2; EPM4; HLGP85; LGP85; LIMP-2; LIMPII; SR-BII
Note	This LIMPII/lpg85 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot, where a band is observed at ~85 kDa. Use in IHC-P was reported in scientific literature (PMID: 23863627).
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Lysosome

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