ABCG1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, IHC, Immunoblotting, IP, WB
Recommend Dilution	Immunoprecipitation, In vivo assay, Flow (Intracellular), Immunohistochemistry: 1:100-1:400, Immunohistochemistry-Paraffin: 1:100-1:400, Immunoblotting, In vitro assay, Western Blot: 1:500
Reactivity	Human, Mouse, Rat, Hamster
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to an internal region of human ABCG1 (between residues 300-400). [UniProt# P45844]
Formulation	Tris-citrate/phosphate, pH 7, 0.1% Sodium azide. Store at 4C. Do not freeze.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	ATP binding cassette subfamily G member 1
Database Link	NP_004906 Entrez Gene 11307 MouseEntrez Gene 85264 RatEntrez Gene 9619 Human UniProt ID P45844
Background	ABCG1 (ATP-binding cassette sub-family G member 1), a transporter protein primarily involved in macrophage lipid homeostasis, localizes to intracellular compartments associated with ER /Golgi membranes, and its expression is highest in macrophage-rich tissue such as spleen, lung, and thymus as well as in brain. ABCG1 form heterodimers with heterologous ABCG partners and functional heterodimerization with ABCG4 has been of great interest because brain cells, mainly neurons and astrocytes, depict high expression of both transporters. ABCG1 involves in the export of a variety of lipids (in conjunction with ABCA1) including cholesterol and phospholipids from macrophages to HDL. Macrophage lipid export is the first step in the reverse cholesterol transport pathway that exports cholesterol from peripheral cells to circulating lipoproteins for eventual excretion via liver. Cholesterol export to lipoprotein acceptors is compromised in macrophage foam cells, one of the early hallmarks of atherosclerosis. Moreover, ABCG1 plays role in T cells/inflammation, brain lipid homeostasis and its expression increases in macrophages of Tangier disease patients.
Synonyms	ABC8; WHITE1
Note	This ABCG1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western Blot. In Western blot mouse peritoneal macrophages treated with 22-R-hydroxycholesterol and T0901317 (stimulating ABCG1 expression) were tested, where a band was observed at ~90 kDa. Preliminary results suggest that this antibody does not appear to work in Immunoprecipitation.In vivo assay was reported in scientific literature.
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	ABC transporters

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