UNG Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, WB
Recommend Dilution	Immunohistochemistry, Immunocytochemistry/ Immunofluorescence: 1:10-1:500, Immunohistochemistry-Paraffin: 10 ug/ml, Western Blot: 2 ug/ml
Reactivity	Human, Mouse, Rat, Rabbit
Host	Rabbit
Clonality	Polyclonal
Immunogen	This antibody was generated by immunizing rabbit with a synthetic peptide sequence CRHFSKTNELLQKSGKKP corresponding to amino acids 281-298 of human UNG1 (NP 003353.1) and amino acids 290-307 of human UNG2 (NP 550433.1). The peptide sequence used for imm
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	uracil DNA glycosylase
Database Link	NP_550433 Entrez Gene 22256 MouseEntrez Gene 304577 RatEntrez Gene 7374 Human UniProt ID P13051
Background	The human UNG gene encodes both nuclear (UNG2) and mitochondrial (UNG1) forms of uracil-DNA glycosylase. These forms are generated by alternative splicing and the use of two differentially regulated promoters, PA and PB (Slupphaug et al., 1993; Nilsen et al., 1997) The cDNAs for UNG1 and UNG2 are of similar size (2061 and 2058 bp, respectively) and as a result the corresponding mRNAs are not resolved as two species in gel electrophoresis (Huag et al., 1998). Nuclear UNG2 differs from mitochondrial UNG1 in 44 amino acids of the N-terminal sequence that is not necessary for catalytic activity. A major role of the UNG gene products is to repair mutagenic U:G mispairs caused by cytosine deamination. For example, UNG2 removes misincorporated dUMP residues. The level and expression pattern of UNG1 and UNG2 differs between cell and tissue type (Huag et al. 1998). Additionally, the expression of UNG is cell cycle regulated (Nagelhus et al. 1995). The expression and activity of UNG has been found in general to be higher in proliferating as compared to nonproliferating tissues and cells (reviewed in Kruman et al. 2004). UNG1 is a 304 amino acid protein. UNG2 is a 313 amino acid protein.
Synonyms	DGU; HIGM4; HIGM5; UDG; UNG1; UNG2; UNG15
Note	Western Blot: Predicted molecular weight 36.5 kDa. Immunocytochemistry/Immunofluorescence See Yang et al (2005)
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency
Protein Pathways	Base excision repair, Primary immunodeficiency

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