LCAT Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, WB
Recommend Dilution	Western Blot: 1:1000, Immunocytochemistry/ Immunofluorescence: 1:500
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Carboxyl-terminal histidine-tagged mouse recombinant LCAT purified from the medium of CHO cells overexpressing mouse LCAT cDNA. [UniProt# P16301]
Formulation	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Whole antisera
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	lecithin-cholesterol acyltransferase
Database Link	NP_000220 Entrez Gene 16816 MouseEntrez Gene 3931 Human UniProt ID P04180
Background	Lecithin: cholesterol acyltransferase (LCAT) is the plasma enzyme responsible for most HDL cholesteryl esters in human plasma. In species with cholesteryl ester transfer protein (CETP) it is also responsible for a significant proportion of VLDL and LDL cholesteryl esters. LCAT, through the esterification of cholesterol, plays a role in the reverse cholesterol transport pathway by facilitating the net movement of cholesterol from peripheral tissues back to the liver for excretion. LCAT deficiency results in low levels of plasma HDL, whereas a transgenic overexpression of LCAT results in increased plasma HDL concentrations.
Synonyms	lecithin-cholesterol acyltransferase
Note	This LCAT antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~62-65 kDa representing LCAT. Also has been shown to immunodeplete LCAT from mouse plasma, suggesting it should work fine for Immunoprecipitation (PMID: 18583707).
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Glycerophospholipid metabolism

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