MAP1LC3A Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, ICC/IF, IHC, Simple Western, WB
Recommend Dilution	Immunohistochemistry-Frozen, Flow Cytometry: 1:100, Immunocytochemistry/ Immunofluorescence: 1:100, Western Blot: 1:2500, Simple Western: 1:40, Immunohistochemistry: 1:100 - 1:400, Immunohistochemistry-Paraffin: 1:100 - 1:400
Reactivity	Human, Mouse, Rat, Bovine, Zebrafish
Host	Rabbit
Clonality	Polyclonal
Immunogen	Genomic sequence made to an N-terminal portion of the human LC3A protein [Swiss-Prot# Q9H492].
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	14 kDa
Gene Name	microtubule associated protein 1 light chain 3 alpha
Database Link	NP_115903 Entrez Gene 66734 MouseEntrez Gene 362245 RatEntrez Gene 84557 Human UniProt ID Q9H492
Background	LC3 (microtubule-associated protein light chain 3), the most studied autophagy biomarker, was originally identified as a subunit of microtubule-associated proteins 1A and 1B (MAP1LC3) and was later found to contain similarity to yeast protein Apg8/Aut7/Cvt5. Distributed ubiquitously in eukaryotes, LC3 is expressed as 3 splice variants/isoforms (LC3A, LC3B and LC3C) which undergo post-translational processing, wherein, the unprocessed form of LC3 is proteolytically cleaved by Atg4 protease to form LC3-I with carboxyterminal exposed glycine. During autophagy, this exposed glycine of LC3-I is conjugated by Atg7 (an E1-like activity), Atg3 (an E2-like conjugating activity) and by Atg12-Atg5-Atg16L multimers (E3-like ligase activity) to phosphatidylethanolamine (PE) moiety for generating LC3-II. The lipophilic character of PE group facilitates LC3-II insertion into autophagosomes membranes, and as a result LC3-II is degraded when autophagosomes fuse with lysosomes to form autolysosomes for lysus of intra-autophagosomal components by lysosomal hydrolases. Conversion of LC3I to LC3II when correlated with autophagosome numbers is considered as the best marker of autophagy because LC3-II is the only well-characterized protein which specifically localize to autophagic structures throughout autophagy (from phagophore to lysosomal degradation). LC3 is a great tool in research as autophagy is implicated in numerous physiological/pathological processes including responses to exercise/aging, cancer, metabolic and neurodegenerative disorders, and cardiovascular/pulmonary diseases.
Synonyms	ATG8E; LC3; LC3A; MAP1ALC3; MAP1BLC3
Note	This LC3 antibody is useful for Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Flow Cytometry and Western blot. In WB a band is seen at ~14 kDa. IHC was done on formalin fixed paraffin embedded sections. In ICC/IF, autophagosome formation has been seen in HeLa cells after treatment with 50uM chloroquine. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 23936035)
Reference Data

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