BMP2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, WB
Recommend Dilution	Immunocytochemistry/ Immunofluorescence, Immunohistochemistry: 1:50-1:200, Western Blot: 0.5 ug/ml, Immunohistochemistry-Paraffin: 1:50-1:200
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to an internal region of human BMP2 (within residues 250-350) [Swiss-Prot# P12643]
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	44 kDa
Gene Name	bone morphogenetic protein 2
Database Link	NP_001191 Entrez Gene 12156 MouseEntrez Gene 29373 RatEntrez Gene 650 Human UniProt ID P12643
Background	Bone morphogenic protein 2 is required for effective healing of fractures in bone. It is necessary for the expression of transcription factors that regulate osteogenesis and differentiation of progenitor cells into mature osteoblasts. Embryologically, BMP2 is important in mesenchymal maturation to fetal valves. The same mechanism of maturation is also utilized by tumor molecular machinery once mesenchymal stem cells are recruited to tumor microenvironments, leading to many recent attempts to generate BMP inhibitors in the treatment of ovarian and colon cancer. The activity of BMP2 is antagonized by the activity of Bambi and Crim1. BMP2 is an interesting target for distinguishing the mechanism by which normal and necessary maturation processes are mutated in tumorigenesis.
Synonyms	BDA2; BMP2A
Note	This BMP2 antibody is useful for Immunohistochemistry-Paraffin and Western Blot, where a band is seen ~44 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data
Protein Families	Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Secreted Protein, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transmembrane
Protein Pathways	Acute myeloid leukemia, Basal cell carcinoma, Cytokine-cytokine receptor interaction, Endocytosis, Hedgehog signaling pathway, Hematopoietic cell lineage, Melanogenesis, Pathways in cancer, TGF-beta signaling pathway

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