FER Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, WB
Recommend Dilution	Immunocytochemistry/ Immunofluorescence: 1:500, Western Blot: 0.5 ug/ml, Knockdown Validated: 0.5 ug/ml
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide made to an internal portion of human FER (within residues 400-450). [Swiss-Prot# P16591]
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	94 kDa
Gene Name	FER tyrosine kinase
Database Link	NP_005237 Entrez Gene 2241 Human UniProt ID P16591
Background	FER (Feline sarcoma related) is a ubiquitously expressed non-receptor protein tyrosine kinase with about 94-kD size that acts downstream of cell surface receptors for growth factors and plays a key role in regulating actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. It acts downstream of EGFR, KIT, FCER1, PDGFRA/PDGFRB receptor etc and phosphorylates CTTN, CTNND1, PTK2/FAK1, GAB1, PECAM1, PTPN11, JUP, PTPN1 and STAT3. Similar to other tyrosine kinases, FER has a central Src-homology 2 (SH2) domain involved in binding to phosphotyrosine-containing peptide sequences and a highly conserved C-terminal kinase domain, whereas it is different by an NH2-terminal FER/ClP4 homology and adjacent coiled-coil domains. FER is expressed ubiquitously in human tissues and higher FER levels are observed in cancer cells compared with adjacent normal cells e.g. renal cell carcinoma.
Synonyms	FerT; Pe1Fe3; Pe1Fe6; Pe1Fe10; Pe1Fe13; PPP1R74; TYK3
Note	This FER antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen ~94 kDa. In ICC/IF cytoplasmic staining was observed.
Reference Data
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Adherens junction

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