GLUT4 (SLC2A4) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, ICC/IF, IHC, WB
Recommend Dilution	Flow (Intracellular), Immunohistochemistry: 1:200, Immunocytochemistry/ Immunofluorescence: 1:100, Western Blot: 0.5ug/ml, Immunohistochemistry-Paraffin, Flow Cytometry
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to a C-terminal portion of the human Glucose Transporter GLUT4 protein (between residues 480-509) [UniProt P14672]
Formulation	PBS, 30% glycerol, 0.1% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	solute carrier family 2 member 4
Database Link	NP_001033 Entrez Gene 20528 MouseEntrez Gene 6517 Human UniProt ID P14672
Background	Glucose Transporter GLUT4 (SLC2A4 or GLUT4) is an insulin-sensitive glucose transporter which facilitate insulin-stimulated glucose uptake in adipose tissue, skeletal muscle and cardiac tissues (that specifically express GLUT4). GLUT4 is a 12-transmembrane domain/ multi-pass membrane protein localized in endomembrane system as well as cytoplasm near perinuclear region, and facilitates glucose transport in the direction of glucose gradient. This transporter localizes to intracellular organelles (endosomes) in unstimulated cells and translocates to the cell surface following insulin stimulation via TBC1D4 phosphorylation. Transgenic mice lacking or overexpressing GLUT4 respectively decrease or increase whole-body insulin sensitivity, suggesting its role in maintenance of glucose homeostasis, and accordingly, under obesity conditions, reduction of GLUT4 gene expression is directly related to insulin resistance development. Inflammatory cytokines produced by the adipose tissue including TNF-alpha, IL-6 etc have been related to reduce GLUT4 expression and defective GLUT4 is an underlying cause of NIDDM.
Synonyms	GLUT4
Note	This GLUT4 antibody is useful for Western blot, IHC and ICC/IF. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24339864). Flow cytometry data from customer review.
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Adipocytokine signaling pathway, Insulin signaling pathway, Type II diabetes mellitus

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