KDM3A / JHDM2A (KDM3A) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, WB
Recommend Dilution	Immunohistochemistry: 1:50, Immunocytochemistry/ Immunofluorescence: 1:500-1:2000, Western Blot: 1:5000, Immunohistochemistry-Paraffin: 1:50
Reactivity	Human, Mouse, Primate
Host	Rabbit
Clonality	Polyclonal
Immunogen	A genomic peptide made to an internal region of the human JMJD1A protein (within residues 250-400). [Swiss-Prot Q9Y4C1]
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	lysine demethylase 3A
Database Link	NP_001140160 Entrez Gene 104263 MouseEntrez Gene 55818 Human UniProt ID Q9Y4C1
Background	JMJD1A is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. It preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. JMJD1A is involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. It's also Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
Synonyms	JHDM2A; JHMD2A; JMJD1; JMJD1A; TSGA
Note	This JMJD1A antibody is useful for immunohistochemistry on paraffin tissues, immunocytochemistry/immunofluorescence and Western blot where a band is seen ~147 kDa. In ICC/IF, nuclear signal was observed in MCF-7 cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data

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