SIRT1 Mouse Monoclonal Antibody [Clone ID: 1F3]

Specifications

Product Data
Clone Name	1F3
Applications	ELISA, FC, ICC/IF, IHC, WB
Recommend Dilution	Flow (Intracellular), Immunohistochemistry Free-Floating, Flow Cytometry: 1 ug per million cells, ELISA: 1:10000, Immunohistochemistry: 1:200-1:1000, Immunocytochemistry/ Immunofluorescence: 1:10-1:1000, Immunohistochemistry-Paraffin: 1:200-1:1000, Western Blot: 1:500-1:2000
Reactivity	Human, Mouse, Rat, Primate
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of human SIRT1 expressed in E. coli. [UniProt# Q96EB6]
Formulation	PBS, 0.03% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Ammonium sulfate precipitation
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	120 kDa
Gene Name	sirtuin 1
Database Link	NP_036370 Entrez Gene 93759 MouseEntrez Gene 309757 RatEntrez Gene 23411 Human UniProt ID Q96EB6
Background	SIRT1, the human homolog of the S. cerevisiae Sir2 protein, functions as an NAD-dependent deacetylase of a number of nonhistone substrates including p53. In response to DNA damage, SIRT1 binds and deacetylates the p53 protein at c-terminal Lys382 residue and attenuate p53-mediated functions. When overexpressed in mouse embryo fibroblasts, SIRT1 antagonizes PML-induced acetylation of p53 and rescues PML-mediated premature cellular senescence. In mammalian cells, SIRT1 appears to control the cellular response to stress by regulating the FOXO family of forkhead transcription factors.
Synonyms	SIR2; SIR2alpha; SIR2L1
Note	This SIRT1 (1F3) antibody is useful for Western blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and ELISA.
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency, Transcription Factors

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