Oct4 (POU5F1) Mouse Monoclonal Antibody [Clone ID: 7E7]

Specifications

Product Data
Clone Name	7E7
Applications	ELISA, FC, ICC/IF, WB
Recommend Dilution	Flow Cytometry: 1:200-1:400, ELISA: 1:10000, Immunocytochemistry/ Immunofluorescence: 1:200-1:1000, Western Blot: 1:500-1:2000
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide derived from an internal portion of human OCT4. [UniProt# Q01860]
Formulation	Preservative: 0.05% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Ascites
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	45 kDa
Gene Name	POU class 5 homeobox 1
Database Link	NP_002692 Entrez Gene 5460 Human UniProt ID Q01860
Background	The OCT4 gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. (provided by RefSeq).Tissue specificity: Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
Synonyms	Oct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4
Note	This OCT4 (7E7) antibody is useful for Western Blot, ELISA, Flow Cytometry and Immunocytochemistry/Immunofluorescence.
Reference Data
Protein Families	Adult stem cells, Cancer stem cells, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency, Transcription Factors

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