KDM3A / JHDM2A (KDM3A) Mouse Monoclonal Antibody [Clone ID: 1E12]

Specifications

Product Data
Clone Name	1E12
Applications	ELISA, ICC/IF, IHC, Simple Western, WB
Recommend Dilution	Immunohistochemistry-Paraffin: 1:200-1:1000, Simple Western: 1:500, ELISA: 1:10000, Immunohistochemistry: 1:200-1:1000, Immunocytochemistry/ Immunofluorescence: 1:10-1:500, Western Blot: 1:500-1:2000
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of human JMJD1A expressed in E. coli. [UniProt# Q9Y4C1]
Formulation	Preservative: 0.05% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Ascites
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	147 kDa
Gene Name	lysine demethylase 3A
Database Link	NP_001140160 Entrez Gene 55818 Human UniProt ID Q9Y4C1
Background	JMJD1A is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. It preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. JMJD1A is involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. It's also Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
Synonyms	JHDM2A; JHMD2A; JMJD1; JMJD1A; TSGA
Note	This JMJD1A (1E12) antibody is useful in Western Blot, ELISA and Immunohistochemistry on paraffin-embedded sections.
Reference Data

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