Mitofusin 1 (MFN1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, WB
Recommend Dilution	Immunohistochemistry: 1:100, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin: 1:100, Western Blot
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A genomic peptide made to an N-terminal region of human Mitofusin-1 (within residues 5-100). [Swiss-Prot Q8IWA4]
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	mitofusin 1
Database Link	NP_284941 Entrez Gene 67414 MouseEntrez Gene 192647 RatEntrez Gene 55669 Human UniProt ID Q8IWA4
Background	Mitochondria are dynamic organelles that continuously divide and fuse, and their dynamics/distribution is critical for their role in bioenergetics and cell survival. Mitochondrial fusion process is mediated by mitofusin 1 (MFN1) and mitofusin 2 (MFN2) proteins in the outer membrane as well as OPA1 in the inner mitochondrial membrane. MFN1 is an essential transmembrane GTPase critical to mitochondrial fusion; acts independently of the cytoskeleton, and its overexpression induces formation of mitochondrial networks. MFN1 forms homo-/-heteromultimers (with MFN2), and the multimerization has been proposed to be essential for mitochondrion fusion. MFN1 interacts with VAT1 and is ubiquitinated by MARCH5. A mutation in the GTPase domain disrupts the mitochondrial fusion activity of MFN1/MFN2 and loss of MFN1/2 leads to mitochondrial dysfunction mediated embryonic lethality in mice. MFN proteins have been reported to regulate mitochondrial membrane potential, while silencing of MFN1/2 resulte in mitochondrial fragmentation and increased apoptotic sensitivity.
Synonyms	hfzo1; hfzo2
Note	This MFN1 antibody is useful for Immunocytochemistry/Immunofluorescence, and IHC-P. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Western Blot was reported in scientific literature.
Reference Data

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