SDHB Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, WB
Recommend Dilution	Immunohistochemistry: 1:50-1:100, Immunocytochemistry/ Immunofluorescence: 1:50-1:100, Western Blot: 1:7000, Immunohistochemistry-Paraffin: 1:50-1:100
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A genomic peptide made to an N-terminal region of the human SDHB protein (within residues 1-150). [Swiss-Prot P21912]
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	succinate dehydrogenase complex iron sulfur subunit B
Database Link	NP_002991 Entrez Gene 67680 MouseEntrez Gene 6390 Human UniProt ID P21912
Background	SDHB [Succinate dehydrogenase (ubiquinone) iron-sulfur subunit, mitochondrial] is an iron-sulfur protein subunit of succinate dehydrogenase (SDH) which is involved in mitochondrial electron transport chain's complex II and is accountable for electrons transfer from succinate to ubiquinone/coenzyme Q. The complex II is a key respiratory enzyme which links Krebs cycle and electron transport chain, and is composed of four subunits: the flavoprotein SDHA, iron-sulfur protein SDHB, and a cytochrome b560 composed of SDHC and SDHD as well as additional groups such as iron-sulphur centres and ubiquinone. SDH complex functions as a tumour suppressor, and loss of any of the subunit proteins leads to destabilization of the complex that follows carcinogenesis. Defects in SDHB have also been linked to susceptibility to pheochromocytoma (PCC), paragangliomas type 4 (PGL4), paraganglioma and gastric stromal sarcoma (PGGSS), and Cowden-like syndrome (CWDLS).
Synonyms	CWS2; IP; PGL4; SDH; SDH1; SDH2; SDHIP
Note	This SDHB antibody is useful for ICC/IF, IHC and Western blot where a band is seen ~31 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Alzheimer's disease, Citrate cycle (TCA cycle), Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease

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