Hsp60 (HSPD1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, ICC/IF, IHC, Immunoblotting, Simple Western, WB
Recommend Dilution	Immunohistochemistry: 1:100, Western Blot: 0.5 ug/ml, Immunohistochemistry-Paraffin: 1:100, Simple Western: 1:5000, Flow Cytometry, Immunoblotting, Knockdown Validated, Immunocytochemistry/ Immunofluorescence: 1:50-1:1000
Reactivity	Human, Mouse, Rat, Hamster, Primate
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to an internal region of the human Hsp60 protein (within residues 70-150). [Swiss-Prot P10809]
Formulation	PBS with 30% Glycerol, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	heat shock protein family D (Hsp60) member 1
Database Link	NP_002147 Entrez Gene 15510 MouseEntrez Gene 63868 RatEntrez Gene 3329 Human UniProt ID P10809
Background	Heat shock protein 60 activates T cells and ultimately autoimmune actions and diseases. In gastric cancer, HSP60 is upregulated in malignant ascites that have been treated, and induces a tumor-specific cytotoxic T lymphocyte response. Enhancing Hsp60 expression is beneficial for protecting bone tissue against osteoblast apoptosis. Hsp60 is highly influenced by dietary intake of antioxidants, and is a prime target for many natural therapies against gut inflammation, preeclampsia, and some low level bacterial infections. Ischemia and reperfusion, as well as hypoxic environments can cause temporary elevations in Hsp60 expression levels.
Synonyms	CPN60; GROEL; HLD4; HSP-60; HSP60; HSP65; HuCHA60; SPG13
Note	This Hsp60 antibody is useful for Immunocytochemistry/Immunofluorescence, Western Blot, and IHC-paraffin embedded sections. In Western Blot, a band is seen ~61 kDa representing Hsp60. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency
Protein Pathways	RNA degradation, Type I diabetes mellitus

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