HSP90AB1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, Simple Western, WB
Recommend Dilution	Knockdown Validated, Simple Western: 1:1000, Immunohistochemistry: 1:200, Immunocytochemistry/ Immunofluorescence: 1:100, Western Blot: 1 ug/ml, Immunohistochemistry-Paraffin: 1:200
Reactivity	Human, Mouse, Rat, Primate
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to a C-terminal portion of the human Hsp90B protein (between residues 650-724) [UniProt P08238]
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	heat shock protein 90kDa alpha family class B member 1
Database Link	NP_031381 Entrez Gene 15516 MouseEntrez Gene 301252 RatEntrez Gene 3326 Human UniProt ID P08238
Background	HSP90AB1 heat shock protein 90kDa alpha (cytosolic), class B member 1 (HSP90AB1), also known as HSPC2, HSPCB and HSP90B, is a chaperone that plays an integral part in signal transduction, protein folding and degradation, cell cycle regulation, and protein maturation. Specifically, HSP90AB1 is a highly conserved chaperone which interacts with other cochaperones to assist in either the folding or refolding of proteins. HSP90AB1 is a constitutive form of the two major HSP90 proteins and is found primarily in the cytosol along with the inducible form, HSP90AA1. HSP90B is a target molecule for treatment of Helicobacter pylori (H. pylori)-induced gastric injury due to an interaction of HSP90B1 with Rac1 ultimately leading to increased production in reactive oxygen species (ROS) in gastric epithelial cells (PMID: 20451655). HSP90B in conjunction with HSP70 also plays a role in HIF-1alpha stability via ubiquitin and O(2)-independent regulation (PMID: 19802386). HSP90 antibodies are useful tools for studying cancer or other diseases brought on by cellular stress.
Synonyms	D6S182; HSP84; HSP90B; HSPC2; HSPCB
Note	This HSP90-1B antibody is useful for Immunocytochemistry/Immunofluorescence, IHC-paraffin embedded sections, and Western blot where a band is seen ~83 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency
Protein Pathways	Antigen processing and presentation, NOD-like receptor signaling pathway, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer

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