XBP1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, ICC/IF, IHC, WB
Recommend Dilution	Western Blot: 1 ug/mL, Flow Cytometry, Knockdown Validated, Immunohistochemistry: 1:100, Immunocytochemistry/ Immunofluorescence: 1:100, Immunohistochemistry-Paraffin: 1:100
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A genomic peptide made to an internal region of the human XBP1 protein (within residues 100-250). [Swiss-Prot P17861]
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	X-box binding protein 1
Database Link	NP_005071 Entrez Gene 22433 MouseEntrez Gene 7494 Human UniProt ID P17861
Background	XBP1 (X-box binding protein 1 or TREB5), is a unique bZIP transcription factor whose active form is generated by a non-conventional splicing reaction upon disruption of homeostasis in endoplasmic reticulum (ER) and unfolded protein response (UPR) activation. Originally identified as a key regulator of MHC class II gene expression in B cells, XBP1 now is known as the most conserved signaling component of UPR and is critical for cell fate determination in response to ER stress. XBP1 is a nuclear transcription factor essential for hepatocyte growth, plasma cells differentiation, immunoglobulin secretion, and UPR cascade. XBP1 acts upon ER stress by activating UPR target genes via direct binding to UPRE (the UPR element). XBP1 binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N3[AT]T-3', and also to certain TPA response elements. XBP1 also binds to HLA DR-alpha promoter as well as to the Tax-responsive element of HTLV-I. XBP1 is up-regulated by ATF6 via direct binding to ERSE in response to ER stress. Genetic variations in XBP1 have been linked with susceptibility to major affective disorder type 7 (MAFD7).
Synonyms	TREB-5; TREB5; XBP-1; XBP2
Note	This XBP1 antibody is useful for ICC/IF and Immunohistochemistry-paraffin embedded sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data
Protein Families	Transcription Factors

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