BNIP3 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, ICC/IF, IHC, WB
Recommend Dilution	Flow Cytometry: 2-5 ug/million cells/ml, Western Blot, Immunohistochemistry: 1:100, Immunocytochemistry/ Immunofluorescence: 1:100, Immunohistochemistry-Paraffin: 1:100
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to an internal region of the human BNIP3 protein (within residues 50-150). [Swiss-Prot Q12983]
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	BCL2/adenovirus E1B 19kDa interacting protein 3
Database Link	NP_004043 Entrez Gene 12176 MouseEntrez Gene 664 Human UniProt ID Q12983
Background	BCL2/adenovirus E1B 19kD protein-interacting protein 3 (BNIP3) is a mitochondrial protein critical for apoptotic events in response to adenovirus infection. It has been implicated in causing a necrotic-like cell death pattern in cells, induced by CD47. This effect has been seen in many cancer cells, and its mis-regulation causes tumor cells to be impervious to normal autophagic events. The tumor suppressor p53 represses the expression of BNIP3, often as a consequence of hypoxic micro-environments as seen in tumors. When BNIP3 is correctly expressed and activated, it translocates to the mitochondria and instigates apoptosis.
Synonyms	NIP3
Note	This BNIP3 antibody is useful for ICC/IF and Immunohistochemistry-paraffin embedded sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data
Protein Families	Druggable Genome, Transmembrane

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