TLR4 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, ICC/IF, IHC, WB
Recommend Dilution	Western Blot: 1:1000, Immunohistochemistry-Paraffin: 1:50, Immunohistochemistry: 1:50, Immunocytochemistry/ Immunofluorescence: 1:50, Flow Cytometry: 2 - 5 ug / million cells
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to the C-terminal portion of the human TLR4 protein (between residues 650-710) [UniProt O00206]
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	toll like receptor 4
Database Link	NP_612564 Entrez Gene 21898 MouseEntrez Gene 29260 RatEntrez Gene 7099 Human UniProt ID O00206
Background	Toll like receptor 4 (TLR4) detects lipopolysaccharide of bacteria. This functionality is critical to activate the innate immune system response. HSP70 expression is enhanced when TLR4 expression is increased, resulting in an impact of the heat shock pathway in inflammation caused by infection. TLR4 binds to lipopolysaccharide and potentiates G-protein coupled receptor signaling, which then induces Neu1 expression. Cell surface expression of Neu1 and TLR4 on macrophages is critical in the ligand activation and inflammation mechanisms. Ultimately, TLR4 can be considered as one of the key elements responsible for inflammatory cytokine release and positive feedback in inflammation.
Synonyms	ARMD10; CD284; TLR-4; TOLL
Note	This TLR4 antibody is useful in Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Pathogenic Escherichia coli infection, Toll-like receptor signaling pathway

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