PLK5 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, WB
Recommend Dilution	Immunohistochemistry: 1:40-1:200, Immunocytochemistry/ Immunofluorescence: 1:40, Western Blot: 1:1000, Immunohistochemistry-Paraffin: 1:40-1:200
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to an internal portion of the human PLK5 protein (between residues 200-250) [UniProt Q496M5]
Formulation	PBS with 30% Glycerol, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	polo like kinase 5
Database Link	NP_001230008 Entrez Gene 216166 MouseEntrez Gene 126520 Human UniProt ID Q496M5
Background	PLK5 (PLK5P, Polo-like kinase 5) is a polo box domain-only protein that is down-regulated in proliferating cells and accumulates in quiescent cells. PLK5 is significantly silenced in brain cancer and overexpression of PKL5 triggers apoptotic effects, both of which suggest that it may have a tumor suppressor function. PLK5 has also been theorized to be important to the proper formation of neurite processes.
Synonyms	PLK-5; PLK5P; SgK384ps
Note	This PLK5 antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and IHC-paraffin embedded sections. In Western Blot, a band is seen ~50 kDa in human brain lysate and ~66 kDa in mouse brain lysate. Molecular weight of PLK5 differs between human and mouse species. In ICC/IF, nuclear staining was observed in HepG2 cells. In IHC-P, nuclear and cytoplasmic staining was seen in mouse salivary gland tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data
Protein Families	Druggable Genome

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