TRPM8 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, WB
Recommend Dilution	Knockdown Validated, Immunocytochemistry/ Immunofluorescence: 1:100, Western Blot: 1:1000, Immunohistochemistry-Paraffin: 1:300, Immunohistochemistry: 1:200
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to an internal portion of the human TRPM8 protein (between residues 250-300) [UniProt Q7Z2W7]
Formulation	PBS with 30% Glycerol, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	transient receptor potential cation channel subfamily M member 8
Database Link	NP_076985 Entrez Gene 171382 MouseEntrez Gene 171384 RatEntrez Gene 79054 Human UniProt ID Q7Z2W7
Background	TRPM8 (TRPP8, LTRPC6) is a transient receptor cation channel that has been shown to be a major cold receptor in sensory neurons. TRPM8 has been theorized to be activated by cold temperatures, voltage, calcium store depletion, some lipids, and also the compounds that produce cooling sensations such as menthol or icilin. TRPM8 is aberrantly over-expressed in pancreatic cancer along with colon, lung, breast and skin cancers, and is proposed to be required for cellular proliferation.
Synonyms	LTRPC6; TRPP8
Note	This TRPM8 antibody is useful for Western Blot where a band is seen ~127 kDa, Immunocytochemistry/Immunofluorescence, and IHC-paraffin embedded sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data
Protein Families	Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane

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