EBI3 Rat Monoclonal Antibody [Clone ID: 10J803]

Specifications

Product Data
Clone Name	10J803
Applications	FC, WB
Recommend Dilution	Flow Cytometry: 1-3 ug/10^6 cells, Western Blot: 0.1-3 ug/ml
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Full-length recombinant mouse EBI3 protein
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	Epstein-Barr virus induced 3
Database Link	NP_005746 Entrez Gene 50498 Mouse UniProt ID Q14213
Background	EBI3 (Epstein-Barr virus Induced-3) is a secreted glycoprotein of the hematopoietin receptor family. It plays a critical regulatory role in the induction of Th2-type immune responses and the development of Th2-mediated tissue inflammation in vivo, which may be mediated through the control of iNKT cell function. EBI3 dimerizes with p28 and p35 subunits of IL-12 to form new proteins IL-27 and IL-35, respectively (Honglian Tong et al, 2010). IL-27 is an early product of activated antigen presenting cell that is produced upon TLR ligation. It negatively regulates Th17 cell differentiation. EBI3 is widely expressed and its expression in dendritic cell is transcriptionally regulated by TLR signaling via MyD88 and NF-kappaB during innate immune responses preceding cytokine driven Th cell development. EBI3 signal inhibits delayed-type hypersensitivity responses by suppressing IL-17 production and inducing IL-10 hyperproduction. EBI3 may play a novel role in controlling tumor metastasis via lung CD8+ T cells, and its deficiency is associated with a diminished production of Th2 cytokines which are known to regulate allergic airway inflammation in asthma. Targeted deletion of EBI3 protects mice from lung metastasis (Kerstin A et al, 2008).
Synonyms	IL-27B; IL27B
Reference Data
Protein Families	Druggable Genome, Secreted Protein

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