NLRP3 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, ICC/IF, IHC, IP, WB
Recommend Dilution	Flow Cytometry, Immunoprecipitation, Immunohistochemistry-Frozen, Western Blot: 2 - 5 ug/mL, Immunomicroscopy, Immunohistochemistry: 1:10 - 1:50, Immunohistochemistry-Paraffin: 1:10 - 1:50, Knockdown Validated, Immunocytochemistry/ Immunofluorescence
Reactivity	Human, Mouse, Rat, Primate
Host	Rabbit
Clonality	Polyclonal
Immunogen	A portion of amino acids 1-50 of human NALP3 was used as the immunogen
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein A purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	118 kDa
Gene Name	NLR family, pyrin domain containing 3
Database Link	NP_004886 Entrez Gene 216799 MouseEntrez Gene 287362 RatEntrez Gene 114548 Human UniProt ID Q96P20
Background	NALP3 (NACHT, LRR and PYD domains-containing protein 3, or NLRP3) belongs to the NLRP family and it contains one DAPIN domain, seven LRR (leucine-rich) repeats and one NATCH domain. It interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain and is a member of the NALP3 inflammasome complex. The NALP3 complex functions as an upstream activator of NF-kappaB signaling and it plays a role in the regulation of inflammation, the immune response and apoptosis. It is also reported that the NALP3 inflammasome can be activated by pathogen-associated molecular patterns (PAMPs) or endogenous danger-associated molecular patterns (DAMPs). The activation of the NALP3 inflammasome results in proteolytic activation and secretion of cytokines of the interleukin-1 (IL-1) family. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome and neonatal-onset multi-system inflammatory disease (NOMID).
Synonyms	AGTAVPRL; AII; AVP; C1orf7; CIAS1; CLR1.1; FCAS; FCAS1; FCU; MWS; NALP3; PYPAF1
Reference Data
Protein Families	Druggable Genome
Protein Pathways	NOD-like receptor signaling pathway

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