Sonic Hedgehog (SHH) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, WB
Recommend Dilution	Western Blot: 1-2 ug/ml, Immunohistochemistry-Paraffin: 1:200 - 1:400, Immunohistochemistry: 1:200 - 1:400, Immunocytochemistry/ Immunofluorescence: 1:200
Reactivity	Human, Mouse, Rat, Primate
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to an N-terminal portion of the human SHH protein (between residues 1-75) [UniProt Q15465]
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	sonic hedgehog
Database Link	NP_000184 Entrez Gene 20423 MouseEntrez Gene 29499 RatEntrez Gene 6469 Human UniProt ID Q15465
Background	Sonic Hedgehog (SHH), also known as HLP3, HPE3, MCOPCB5, SMMC1, and HHG1, is a gene that encodes for a protein instrumental in patterning the early embryo. Specifically, SHH has been implicated as the main signal in mapping out the development of the ventral neural tube, the anterior-posterior limb access, and the ventral somites. This occurs by SHH binding to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. SHH also regulates another target gene, the gli oncogene. In adults, SHH helps to control stem cell proliferation. The N-terminal form of SHH is associated with lipid rafts at the cell surface, while on a tissue level SHH is expressed in fetal intestine, liver, lung and kidney. Recent research has found SHH to modulate the spatial organization of neuroepithelial cell proliferation in the embryonic chick (PMID: 23031710), as well as detected a role for SHH in Idiopathic Pulmonary Fibrosis (PMID: 23023967).
Synonyms	HHG1; HLP3; HPE3; MCOPCB5; SMMCI; TPT; TPTPS
Note	This SHH antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. In Western blot a band is observed at ~50 kDa. In Immunocytochemistry/Immunofluorescence cytoplasm/membrane staining was observed in SH-SY5Y cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
Protein Pathways	Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer

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