Dopamine Transporter (SLC6A3) Mouse Monoclonal Antibody [Clone ID: mAb16]

Specifications

Product Data
Clone Name	mAb16
Applications	ELISA, ICC/IF, IHC, IP, WB
Recommend Dilution	Immunoprecipitation, Immunohistochemistry-Paraffin: 1:100, ELISA, Immunohistochemistry: 1:100, Immunocytochemistry/ Immunofluorescence: 1:250, Western Blot: 1:1000
Reactivity	Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide corresponding to the N-terminus of rat DAT1. [UniProt# P23977]
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	solute carrier family 6 member 3
Database Link	NP_001035 Entrez Gene 13162 MouseEntrez Gene 24898 RatEntrez Gene 6531 Human UniProt ID Q01959
Background	DAT1, also known as SLC6A3 and Sodium-dependent dopamine transporter, is a sodium and chloride-dependent dopamine transporter belonging to the solute carrier family 6. Dopamine transporters are distributed widely across the brain in regions with high dopaminergic activity, such as the striatum and substantia nigra. These transporters effectively provide fast acting clearance of dopamine, adrenalin and noradrenalin from the synaptic cleft, thus terminating the neurotransmitter signal. DATs have been found to interact with PRKCABP, TGFB1I1, SLC18A2 and the N-terminus of SYNGR3. DATs are also the target proteins for psychomotor stimulants such as amphetamines and cocaine. Research has implicated DATs as useful markers in detecting depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999).
Synonyms	DAT; DAT1; PKDYS
Note	This DAT1 (mAb16) antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and ELISA. In WB a band can been seen at ~70-85 kDa. Use in Immunoprecipitation reported in scientific literature (PMID: 20643191).
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Parkinson's disease

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