GAPDH Mouse Monoclonal Antibody [Clone ID: 13H12]

Specifications

Product Data
Clone Name	13H12
Applications	ICC/IF, IHC, Simple Western, WB
Recommend Dilution	Immunohistochemistry: 5 ug/ml, Immunocytochemistry/ Immunofluorescence: 1:10, Western Blot: 0.25 - 1 ug/ml, Immunohistochemistry-Paraffin: 5 ug/ml, Simple Western: 1:25
Reactivity	Human, Mouse, Drosophila, Primate
Host	Mouse
Clonality	Monoclonal
Immunogen	Amino acids between 275 and 325 of glyceraldehyde 3-phosphate dehydrogenase protein were used as the immunogen for this antibody.
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	glyceraldehyde-3-phosphate dehydrogenase
Database Link	NP_002037 Entrez Gene 14433 MouseEntrez Gene 2597 Human UniProt ID P04406
Background	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export. GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Synonyms	G3PD; GAPD; HEL-S-162eP
Note	GAPDH is a widely used loading control for quantitative Western blotting. In IHC-P, the staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Reference Data
Protein Families	ES Cell Differentiation/IPS
Protein Pathways	Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways

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